Approximately 150,000 animals, primarily mice and rats, are used annually in the European Union for chemical safety testing. Scientists are now exploring artificial intelligence (AI) as a potential solution to significantly reduce this number. Researchers are focusing on employing AI with human cells and “mini-organs” to develop advanced testing methods. These new tests aim to more accurately identify hazardous chemicals and better predict risks to human health. The ultimate goal is to minimize reliance on animal testing while ensuring the safety of commonly used chemicals. This shift represents a promising step towards improved animal welfare and more ethical scientific practices within the EU.