Beginning in 2021, authorities in Wyoming treated 52 miles of the Big Sandy River with rotenone, a substance toxic to fish, to eradicate invasive species. Over 6,000 specimens of *Catostomus* were relocated to conservation tanks at a Wyoming state laboratory during the process. The treatment proved successful, allowing for the reintroduction of 2,109 native *Catostomus latipinnis* into the restored section of the Big Sandy River by 2024. Biologists initiated the project to improve the health of the Big Sandy River ecosystem. The use of rotenone was a critical step in removing competing, non-native fish populations. This project demonstrates a successful strategy for river restoration through targeted species removal and reintroduction efforts in Wyoming.